September 8th, 2025 7:00am
Below is the D2Football.com Top 25 poll folllowing week one. Johnson C. Smith and Colorado Mines entered the poll for the first time this fall, while Fort Hays rejoins the rankings after an impressive win at CSU Pueblo. Charleston, Ouachita Baptist and Valdosta State dropped from the poll this week.
| Rank | Team | Record | Previous Rank |
| 1 | Ferris State | 2-0 | 1 |
| 2 | Harding | 1-0 | 2 |
| 3 | Minnesota State | 2-0 | 3 |
| 4 | Kutztown | 1-0 | 4 |
| 5 | Grand Valley State | 2-0 | 5 |
| 6 | Western Colorado | 2-0 | 7 |
| 7 | West Florida | 2-0 | 8 |
| 8 | Angelo State | 2-0 | 10 |
| 9 | Slippery Rock | 1-0 | 9 |
| 10 | UT Permian Basin | 2-0 | 16 |
| 11 | Augustana | 2-0 | 15 |
| 12 | Central Oklahoma | 1-1 | 17 |
| 13 | CSU Pueblo | 1-1 | 6 |
| 14 | California | 1-0 | 18 |
| 15 | Virginia Union | 1-0 | 19 |
| 16 | Pittsburg State | 0-2 | 12 |
| 17 | Lenoir-Rhyne | 1-1 | 20 |
| 18 | West Alabama | 2-0 | 22 |
| 19 | Delta State | 2-0 | 24 |
| 20 | Ashland | 1-0 | 25 |
| 21 | Central Washington | 1-1 | 23 |
| 22 | Fort Hays | 1-1 | NR |
| 23 | Johnson C Smith | 2-0 | NR |
| 24 | Colorado Mines | 1-0 | NR |
| 25 | Indianapolis | 1-1 | 13 |